Fig. 6.
Effect of MEK inhibitor PD98 059 on ERK2 activation in eotaxin-stimulated eosinophils.
Eosinophils were preincubated with medium or 0.5, 5.0, or 50 μM of PD98 059 for 30 minutes and stimulated with medium or eotaxin (10−8 mol/L) for 1 minute. Cytosolic extracts underwent Western blotting with the anti-phospho-ERK1/ERK2 antibody (A). The membrane was then stripped and reprobed with the anti-ERK2 antibody (B). PD98 059 blocked ERK1/2 phosphorylation in a dose-dependent manner (n = 4).
