Migration of PMCs in response to NGF.
(A) PMCs (5 × 104 cells/500 μL) placed in culture inserts (upper compartment) were allowed to migrate toward 50 ng/mL NGF (closed) or medium alone (open) in each well of 24-well culture plates (lower compartment). Mast cell migration was assessed by counting the number of PMCs through the polycarbonate membrane. Each point represents the mean ± SE (standard error) of 4 separate experiments. (B) Various concentrations of NGF were applied into the lower compartment. After incubation for 3 hours, migratory cells were counted. Each point represents the mean ± SE of 4-11 separate experiments. (C) Anti-NGF (1:500 dilution), anti-SCF (5 μg/mL), or control pAb was added into 50 ng/mL NGF or 1 ng/mL rSCF in the lower compartment. After incubation for 3 hours, migratory PMCs were counted. Data were obtained from 5-9 separate experiments. *P < .001, when compared with NGF or rSCF pAb and control pAb.
