3T3 fibroblast-derived chemotactic activity.

(A) PMCs placed in culture inserts were allowed to migrate toward the monolayer of 3T3 fibroblasts unfixed (closed circle) or fixed with paraformaldehyde (open triangle) or medium alone (open circle) in each well of 24-well culture plates. After incubation for various hours, migratory cells were counted. Each point represents the mean ± SE of 4-11 separate experiments. (B) Anti-NGF (1:500 dilution) and/or anti-SCF pAb (40 μg/mL) or control pAb was added into the monolayer of 3T3 fibroblasts in each well of 24-well culture plates. After incubation for 24 hours, migratory cells were counted. Data were obtained from 5-9 separate experiments. *P < .001, when compared with fibroblasts and control pAb.