Reconstitution analysis of the mice transplanted with day 12-fetal liver cells at 20 weeks after transplantation.

The representative FACS profile of peripheral blood analysis is shown. The reconstitution in tri-lineage: myeloid, B cell, and T cell was a criterion for engraftment. T: test donor cells (fetal liver cells derived from F1 mouse), C: competitor cells (bone marrow cells from B6-Ly5.1), and R: residual host cells from B6-Ly5.2. T/C ratio: a ratio of (% test donor-derived cells)/ (% competitor-derived cells). In this case, T = 45.0%, C = 44.0%, R = 1.0%, and T/C ratio = 1.02.