Fig. 2.
Fig. 2. Collagen type I inhibits AICD in Jurkat cells. / (A) Jurkat cells were stimulated with or without immobilized anti-CD3 antibody for 24 hours in the presence or absence of 50 μg/mL of the indicated ECM proteins. Apoptosis was determined by DNA fragmentation analysis. Fn, fibronectin; Ln, laminin; Coll, collagen I. *P < .05 between anti-CD3-stimulated control and collagen-treated samples. (B) Cells were stimulated with or without PMA/ionomycin for 24 hours in the presence or absence of 50 μg/mL of collagen I (Coll I) or poly-l-lysine (PLL). Cell death was determined by propidium iodide uptake as described in “Materials and methods.” *P < .05 between PMA/ionomycin-stimulated control and collagen-treated samples. (C) Dose-response effect of collagen I on AICD. The results are representative of 3 independent experiments.

Collagen type I inhibits AICD in Jurkat cells.

(A) Jurkat cells were stimulated with or without immobilized anti-CD3 antibody for 24 hours in the presence or absence of 50 μg/mL of the indicated ECM proteins. Apoptosis was determined by DNA fragmentation analysis. Fn, fibronectin; Ln, laminin; Coll, collagen I. *P < .05 between anti-CD3-stimulated control and collagen-treated samples. (B) Cells were stimulated with or without PMA/ionomycin for 24 hours in the presence or absence of 50 μg/mL of collagen I (Coll I) or poly-l-lysine (PLL). Cell death was determined by propidium iodide uptake as described in “Materials and methods.” *P < .05 between PMA/ionomycin-stimulated control and collagen-treated samples. (C) Dose-response effect of collagen I on AICD. The results are representative of 3 independent experiments.

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