Effects of CTX injection on the expression of Ly-6C antigen and on BrdU incorporation in spleen lymphocytes at different times after treatment.

Seven- to 8-week-old DBA/2 mice were placed on BrdU water from day 0 to sacrifice. Mice were injected intraperitoneally with CTX (83 mg/kg) (dotted bars) or saline (white bars) at day 0. At different time intervals, spleens were taken and disaggregated. Cells were stained for Ly-6C; this was followed, after fixation, by nuclear staining for BrdU incorporation. Cell fluorescence was evaluated by fluorescence-activated cell sorter (FACS). There were 3 mice per group. The data show the mean (± SE) of the percentage of Ly-6C^hi cells (upper panel) or BrdU^hi cells (lower panel) with respect to the total number of spleen lymphocytes.