Effects of injection of anti–IFN-/β antibodies on the CTX-induced up-regulation of Ly-6C expression in spleen lymphocytes from mice.
Seven- to 8-week-old DBA/2 mice were injected intraperitoneally with CTX (83 mg/kg) (dotted bars) or saline (white bars) at day 0. One day before and 2 and 4 days after CTX treatment, some mice were also injected intraperitoneally with 0.2 mL of an anti–murine IFN-α/β antibody preparation (160 000 IFN-neutralizing units/mouse/injection) (striped bars). At different time intervals, spleens were collected and cells were stained for Ly-6C expression and processed for FACS analysis. There were 3 mice per group. The data show the mean (± SE) of the percentage of Ly-6Chi cells with respect to the total number of spleen lymphocytes.
