Lymphocyte subpopulation analysis in CD69 (-/-) and wild-type mice.

Cells from lymphoid organs were obtained from CD69 (-/-) mice and wild-type littermate controls, then immunofluorescently stained and analyzed using flow cytometry. (A, B) CD3 expression on thymocytes and CD4 and CD8 markers on thymocytes, splenocytes, and lymph node cells. Numbers indicate the percentage of lymphoid populations. Data from a single mouse are shown, they are representative of more than five mice per group and from more than one litter. (C) Immunochemical analysis of lymphoid organs from CD69 (-/-) mice. Frozen sections of lymph nodes and spleens from unimmunized mice were immunostained for immunoglobulin M. Peyer's patches obtained from mice 10 days after challenge by intraperitoneal injection of 10 μg of alum absorbed 2,4-dinitrophenyl-keyhole limpet hemocyanin mice were stained with anti-CD5.