Fig. 4.
Fig. 4. Analysis of thymic selection and in vivo proliferation of mature T cells in CD69 (-/-) F5 T-cell receptor (TCR) transgenic mice. / (A) Two-color flow cytometry analysis was performed in thymocytes of F5 TCR transgenic mice untreated or after the administration of NP366–374 as described in Materials and methods section. (B) The proliferative capability of mature T cells in CD69 (-/-) mice was analyzed in vivo after bromodeoxyuridine (BrdU) treatment as in the Materials and methods section. Numbers indicate the percentage of positive, BrdU-incorporating lymph node cells. Data shown are representative of results obtained in three mice per group and from more than one litter.

Analysis of thymic selection and in vivo proliferation of mature T cells in CD69 (-/-) F5 T-cell receptor (TCR) transgenic mice.

(A) Two-color flow cytometry analysis was performed in thymocytes of F5 TCR transgenic mice untreated or after the administration of NP366–374 as described in Materials and methods section. (B) The proliferative capability of mature T cells in CD69 (-/-) mice was analyzed in vivo after bromodeoxyuridine (BrdU) treatment as in the Materials and methods section. Numbers indicate the percentage of positive, BrdU-incorporating lymph node cells. Data shown are representative of results obtained in three mice per group and from more than one litter.

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