Hexamethylene bisacetamide (HMBA)-stimulated activation of caspase 9.

CCRF and A7 CEM cells were treated with (A) 0-15 mmol/L HMBA for 72 hours or (B) 100 ng/mL anti-Fas antibody (CH-11) for 12 hours. Protein lystates were separated by SDS/PAGE, and Western blots were performed using anti-caspase-3 (Caspase 3), anti-caspase-8 monoclonal antibody (Caspase 8), and anti-caspase-9 polyclonal antibody (Caspase 9). The inactive (pro) and active (cleaved) forms of caspases-3, -8, and -9 are indicated. Nonspecific protein bands are denoted by an asterisk.