Testosterone modulates AR expression in HEL cells.

HEL cells were grown for 48 hours in 10% FBS, with or without testosterone, and stained for AR as described above. Cells were incubated with 0 nmol/L (panel A), 1 nmol/L (panel B), 5 nmol/L (panel C), 10 nmol/L (panel D), and 100 nmol/L (panel E) testosterone. Testosterone requires ethanol to go into suspension, and all experiments had ethanol at a final concentration of 0.001%. The reduction in AR in cells treated with ethanol alone (panel A) is expected.37 Original range of magnifications: ×800 to ×2400.