Cell adhesion.

(A) Cell adhesion of lymphoma cell lines to bovine serum albumin or FN coated at 10 μg/mL was carried out in the presence of 0.3 mmol/L Mg²⁺ or 20 μmol/L Mn²⁺. The values reported represent the average of 3 experiments. (B) Cell adhesion to endothelial cells. Confluent H-end80 monolayers were incubated with media alone or with media containing TNF-α for 16 hours and rinsed with DMEM. NQ22 and NQ29 cells (2 × 10⁵) in DMEM were added for 20 minutes at 37°C under static conditions. At the end of the incubation period, the nonadherent cells were removed by gentle rinsing with DMEM and the percentage of bound cells was determined as detailed in “Materials and methods.” (C) NQ22 and NQ29 cell adhesion to purified VCAM-1 or bovine serum albumin coated at 10 μg/mL. For the inhibition of cell attachment to H-end80 cells and to purified VCAM-1, the different antibodies were added at 5 μg/mL just before the lymphoma cells were plated. The antibodies used were the following: DATK-32 (α4β7); M1/70 (Mac-1/αMβ2); PS/2 (α4); M298 (β7); and 429 (VCAM-1).