Inhibition of VCAM-1 induction by TNF--neutralizing antibodies.

A polyclonal antibody (10 μg/mL) against TNF-α (A) or a mixture of polyclonal antibodies (3.3 μg/mL each antibody) against IFN-γ, GM-CSF, and TNF-α (B) were added to H-end80 cells during the coculture with NQ22 cells. The expression of VCAM-1 was assessed with mAb 429; basal, basal expression of VCAM-1 in H-end80 cells.