American Society of Hematology

Fig. 4.

$Fig. 4. Association of ICAM-3 and moesin in neutrophils. / ICAM-3 was isolated from human neutrophil lysates by antibody-affinity chromatography. (A) A polypeptide of 78 kd (arrow) eluted in fractions at pH 11.0 (lanes 1-2) while ICAM-3 coeluted at pH 11.0 (lane 2) and separated from the 78-kd polypeptide at pH 11.5 (lanes 3-4). (B) Western blot analysis of purified recombinant ezrin (lane 1) and the pooled ICAM-3 fraction (lane 2) with ezrin antibodies and the same pooled ICAM-3 fraction (lane 3) and recombinant moesin (lane 4) with moesin antibodies. A strong signal for moesin showed that the 78-kd polypeptide coeluting with ICAM-3 (Figure 4A) corresponded to moesin.$

Association of ICAM-3 and moesin in neutrophils.

ICAM-3 was isolated from human neutrophil lysates by antibody-affinity chromatography. (A) A polypeptide of 78 kd (arrow) eluted in fractions at pH 11.0 (lanes 1-2) while ICAM-3 coeluted at pH 11.0 (lane 2) and separated from the 78-kd polypeptide at pH 11.5 (lanes 3-4). (B) Western blot analysis of purified recombinant ezrin (lane 1) and the pooled ICAM-3 fraction (lane 2) with ezrin antibodies and the same pooled ICAM-3 fraction (lane 3) and recombinant moesin (lane 4) with moesin antibodies. A strong signal for moesin showed that the 78-kd polypeptide coeluting with ICAM-3 (Figure 4A) corresponded to moesin.

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