Figure 4.
Gel electrophoresis with or without stacking gel followed by Western blot analysis of IκBα32/36A. MS4A-IκBα32/36A-transfected cells were (lanes 1 and 3) or were not (lanes 2 and 4) treated with doxycycline for 48 hours. SDS–PAGE and Western blot detection of IκBα was performed without (lanes 1 and 2) or with (lanes 3 and 4) stacking gel.