SSCP of PCR amplified genomic DNA strands of exon 2.

SSCP was performed on genomic DNA samples from normal donors (n1, n2, and n3) and A47° CGD patients (p1, p2, and p3) using the nested primer pairs 2LA/2LB and 2RC/2RD. The amplification products containing the 3′ end of intron 1 and the 5′ end of exon 2 were electrophoresed on a nondenaturing polyacrylamide gel. The figure shows a typical SSCP analysis, where the diagnostic band (−>) was missing in all patients carrying ΔGT.