Effects of VEGF on Ter119⁺ and CD45⁺ EB cell generation are concentration dependent. Both A3-1 and E14 ES cells were differentiated in various concentrations of BMP-4 and VEGF analyzed as in Figures 1 and 3. Generation of Ter119⁺ (R3), CD45⁺ (R7), CD34⁺ CD31^hi (R5), and CD34⁺CD31^lo (R6) cells were quantified as % total EB cells. Except for the results with 0.15 ng/mL BMP4, which were obtained once with each ES cell line, numbers were derived from an average of 2 to 5 independent experiments and were plotted according to the VEGF concentration with the corresponding SD (vertical line). Open symbols are results from A3-1 ES cells, and closed symbols are from E14 ES cells. Squares indicate results from 0 ng/mL BMP-4, triangles indicate results of 0.15 ng/mL BMP-4, circles indicate results from 1.5 ng/mL BMP-4, and the diamond indicates results of 15 ng/mL BMP-4.