Identification of pulmonary antigen-presenting cell (APC) populations under different conditions.
Total mononuclear cells were isolated from mouse lungs that received 0.6 × 109 plaque-forming units adenoviral gene vector expressing murine GM-CSF (AdGM-CSF) or Addl70-3, or 40 μL of sterile phosphate-buffered saline at day 12. Approximately 0.3 × 106 cells were immunostained with FITC-CD11b, PE-CD11c and biotinylated-MHC II, B7.1, or rat immunoglobulin (Ig)G2a antibodies. Data were collected from FACScan. (A) General cellular profile for each group was shown on forward-side scatter, macrophage/monocyte-like cell enriched population was gated as R1. (B) R1 region cells were further divided into three subpopulations in contour plot based on CD11b and CD11c expression. (C) The absolute cell numbers for each population derived from single mouse in different groups. Data are representative of three individual experiments.
