Effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on activation of R2 region CD11b^low/CD11c^bright population during pulmonary adenoviral infection.

Following the procedure described in Figure 1, (A) MHC II and B7.1 expression were examined on R2 region for each group. Open histograms represent the background staining with the isotype control antibody, whereas solid histograms indicate staining with relevant monoclonal antibody against the indicated surface molecule. M1 markers represent the limit defining the expression at high levels of the corresponding marker. In the case of B7.1 expression, the majority of cells from R2 region in control groups expressed at dull level that labeled as M8. (B) The absolute cell numbers for double positive for CD11c and MHC II or B7.1 derived from single mouse in different groups.