Major effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on macrophages at earlier times in the lung.

In a separate experiment, mononuclear cells were isolated from mice receiving phosphate-buffered saline, Addl70-3, or adenoviral gene vector expressing murine GM-CSF (AdGM-CSF) at day 5 and immunostained in the exact same way as described in Figure 1. A parallel comparison analysis was carried out between the AdGM-CSF day 5 group and the AdGM-CSF day 12 group. R1 region cells as defined in Figure 1 were expressed in dot plot based on CD11b and CD11c expression. The number marked on each corner represents the percentage of a given phenotype out of total R1 region.