Fig. 9.
Fig. 9. Interferon (IFN)γ level determined in ex vivo antigen-recall responses. / Mediastinal lymph nodes and lungs were collected from the mice receiving adenoviral gene vector expressing murine granulocyte-macrophage colony-stimulating factor (AdGM-CSF) or Addl70-3 at day 12. T lymphocytes were isolated and cultured at various conditions as described in “Material and methods.” Supernatants of (A) mediastinal lymph node-derived T-cell culture and (B) lung-derived T-cell culture were collected at 72 hours, and IFNγ level was measured by enzyme-linked immunosorbent assay. The difference in the level of IFNγ production between AdGM-CSF and control groups is statistically significant (t test,P ≤ .01). Data are representative of three individual experiments and expressed as mean ± SEM of triplicate samples.

Interferon (IFN)γ level determined in ex vivo antigen-recall responses.

Mediastinal lymph nodes and lungs were collected from the mice receiving adenoviral gene vector expressing murine granulocyte-macrophage colony-stimulating factor (AdGM-CSF) or Addl70-3 at day 12. T lymphocytes were isolated and cultured at various conditions as described in “Material and methods.” Supernatants of (A) mediastinal lymph node-derived T-cell culture and (B) lung-derived T-cell culture were collected at 72 hours, and IFNγ level was measured by enzyme-linked immunosorbent assay. The difference in the level of IFNγ production between AdGM-CSF and control groups is statistically significant (t test,P ≤ .01). Data are representative of three individual experiments and expressed as mean ± SEM of triplicate samples.

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