Fig. 4.
Expression of human F.IX (ng/106 cells/24 hours) in conditioned media after in vitro transduction of cultured human cells (24-hour supernatants) with AAV vector.
MOIs were 104 vector genomes/cell. Results are shown for vectors AAV-CMV-hF.IX-2 (CMV) and AAV-HSA/CMV-hF.IX-2 (HSA/CMV). Cell types were HEK-293 cells, HepG2 cells, and human myotubes derived from primary myoblasts. Cells were transduced in triplicate. Values for myotubes were average for 24-hour supernatants at days 11 and 18 posttransduction; values for HEK-293 and HepG2 cells were average for 24-hour supernatants at day 4 posttransduction. Vertical lines are standard deviation.
