Constitutive activation of LIL-Stat in leukemic cells of patients with ATL.

LILRE was used as a radiolabeled probe. Nuclear extracts were prepared from ATL cells (A, B, and lanes 3 to 10 of C) and LPS-stimulated THP-1 monocytic leukemia cells (lanes 1 and 2 of C). The double-stranded oligonucleotides used in this study were described in “Materials and methods.” Unlabeled competitor oligonucleotides were used at a 50-fold molar excess over radiolabeled LILRE probe. D shows the enhancer activity of LILRE in leukemic cells of 2 patients with ATL. Transfection of CAT reporters into ATL cells and CAT assays were carried out as described in “Materials and methods.” The CAT data were normalized to the average activity elicited by eitherfos/CAT or 3M. Error bars represent SDs from triplicate cultures.