Fig. 2.
Fig. 2. Effect of physiologic concentrations of LPA on the kinetics of Ca++ entry into human RBCs. / Erythrocytes prepared and loaded with Fluo-3, as described in the legend to Figure 1, were treated with (top to bottom tracing) 5 μmol/L LPA, 2 μmol/L LPA, 1 μmol/L LPA, 0.5 μmol/L LPA, or no LPA at time t = 60 seconds. The change in Fluo-3 fluorescence caused by Ca++ entry into the cells was then monitored by fluorescence spectroscopy.

Effect of physiologic concentrations of LPA on the kinetics of Ca++ entry into human RBCs.

Erythrocytes prepared and loaded with Fluo-3, as described in the legend to Figure 1, were treated with (top to bottom tracing) 5 μmol/L LPA, 2 μmol/L LPA, 1 μmol/L LPA, 0.5 μmol/L LPA, or no LPA at time t = 60 seconds. The change in Fluo-3 fluorescence caused by Ca++ entry into the cells was then monitored by fluorescence spectroscopy.

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