Fig. 4.
![Fig. 4. ERK expression and activation in GM-CSF–treated TF-1 cells. / TF-1 cells were serum- and factor-starved for 24 hours, and placed in RPMI with BSA 0.5%. Cells were stimulated with rhGM-CSF (1 nmol/L) for 1, 2, 5, 10, and 20 minutes. TPA-stimulated cells represent the positive control and diluent-stimulated, the negative control (15-minute stimulation). The lysate from 2 × 105cells was separated on a 10% SDS-PAGE gel. Antiphosphorylated ERK antibody ([A], 1:20 000) or anti-ERK antibody ([B], 1:5000) was used to probe each blot. The blots represent samples taken from the same experiment loaded on 2 separate gels.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2552/4/bloo00830004w.jpeg?Expires=1735541520&Signature=zaNwW54a6Xy7GuYK60BRGT7ER7N6lI~yEqaC8gaqtv9eYRdqxQfujxx1dOfN~1VwnpX3hutRERuW44EsCWEMyQwjiO1OXa7~76KQKhYvlb6H5vFYD0nnSGxLeVXeYGKsJ2VMBjdT46qh-WqcLCLTJ2eF~T8QVJJVkTQzve6KxqreCc~wI3RkPVFxxBFNjOHAky5~5XkjutYI1XbWmQmQD31s~ozxntiZW4KoAsA5lEG4c1W7Kl-UKQkufHDx583jxui5TwSSj~4-axI8JaFbdeA9cM63Plyr8ZpI4qhYKQuew4k0uU5~R5fWyf0cGReZ8xzxxxsbO2t~A9zFCy1hxQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ERK expression and activation in GM-CSF–treated TF-1 cells.
TF-1 cells were serum- and factor-starved for 24 hours, and placed in RPMI with BSA 0.5%. Cells were stimulated with rhGM-CSF (1 nmol/L) for 1, 2, 5, 10, and 20 minutes. TPA-stimulated cells represent the positive control and diluent-stimulated, the negative control (15-minute stimulation). The lysate from 2 × 105cells was separated on a 10% SDS-PAGE gel. Antiphosphorylated ERK antibody ([A], 1:20 000) or anti-ERK antibody ([B], 1:5000) was used to probe each blot. The blots represent samples taken from the same experiment loaded on 2 separate gels.
