DC-mediated growth inhibition of human tumor lines in vitro.

DCs at 5 × 10⁴ cells/well were cultured with or without 5 μg/mL of LPS in 96-well flat-bottom plates. At 24 hours, 100 μL of culture medium was removed, and indicated human tumor lines at 1 × 10⁴cells were added to the wells. The cultures were pulsed with³H-TdR for the last 24 hours the DCs were cultured with tumor cells. The results are presented as the percentage of inhibition of tumor-cell proliferation. The results are presented as the mean ± SD of triplicate wells. ³H-TdR incorporation into DC was less than 1500 cpm; 2 (A and B) of 5 representative donors are shown. *P < .05 compared with DCs cultured with medium.