Fig. 6.
Effect of maturation induction in DC-mediated growth inhibition.
DCs (2 × 106 cells/mL) were cultured in polypropylene tubes in the presence of medium, LPS (5 μg/mL), TNF-α (100 ng/mL), IFN-γ (1000 U/mL), or anti-CD40 mAb (10 μg/mL). At 24 hours, DCs were washed 3 times and cultured with HT29 (1 × 104 cells/well) in 96-well plates at an E-to-T ratio of 5:1. Proliferation was measured 48 hours after the start of the incubation of DCs with tumor targets. The results are presented as the mean ± SD of triplicate wells. The results of 3 representative experiments using DCs from different donors is shown. *P < .05 compared with DCs cultured with medium.
