Fig. 8.
Effect of anti–TNF- serum on DC-mediated tumor growth inhibition.
DCs were cultured with or without LPS as described. At 24 hours, DC supernatants (A) or DCs themselves (B) were mixed with HT29 tumor cells and cultured in the presence or absence of anti–TNF-α serum at a final dilution of 1 to 400. The plates were further incubated for 48 hours, and tumor cell proliferation was measured by 3H-TdR incorporation. The results are presented as the mean ± SD of triplicate wells. The results of 3 representative experiments using DCs from different donors are shown. *P < .05 compared with DCs cultured with medium.
