Comparative analysis of membrane PAF-receptor expression on K562 and Daudi cells.

A representative experiment is shown. K562 and Daudi cells were analyzed before and after 10 and 30 minutes of NK cell attack, stained with anti–PAF-receptor mAb detected with phosphatidylethanolamine (PE)–goat antimouse antibody and with FITC-conjugated antiglycophorin (K562) or anti-CD19 (Daudi). Data are displayed as dot plots, and PAF-receptor expression was analyzed only on glycophorin (K562) and CD19 (Daudi) positive cells. Only a limited number of double-labeled and viable target cells could be analyzed (ie, 5.10²), owing to the expected rapid NK-mediated induced–target-cell lysis. Cells that are double-stained with corresponding PE- and FITC-conjugated antibodies are represented in the upper right quadrant.