Fig. 2.
Identification of integrins by flow cytometry.
Neutrophils were incubated in 5 μg/mL of the monoclonal antibodies JB55 (α5) (A), CLB-705 (α5β1) (B), or JB1a (β1) (C) in the presence of fMLP (solid lines). For comparison, cells were incubated with an irrelevant control antibody MOPC21 (short dashes) or with the monoclonal antibody against β2 integrin, IB4 (long dashes). The cells were rinsed with PBS and then incubated in a fluorescein-conjugated secondary antibody. The cells were fixed with paraformaldehyde before flow cytometry. Intensity histograms for each of the monoclonal antibodies are shown. For each condition, 1 × 104cells were measured.
