Fig. 5.
![Fig. 5. [Ca++]i-buffering causes a redistribution of F-actin and talin in PMNs. / PMNs were either loaded with quin2/AM (D-F) or not (A-C), then prepared for immunofluorescence as described. Samples were incubated in 5 μg/mL of a monoclonal antibody to talin, rinsed, and then stained with a TRITC-conjugated secondary antibody and FITC-conjugated phalloidin to visualize F-actin. In control cells (A-C), talin (C) is found colocalized with F-actin (B) predominately at the leading edge of motile cells. In contrast, when cells are [Ca++]i-buffered both talin (F) and F-actin (E) are found at the rear of cells as well as in the leading lamella if one exists. DIC images are shown (A, D). Bar = 10 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2471/4/bloo00726005y.jpeg?Expires=1735847741&Signature=qmBA-89ermh7cU8U6FJ3Djhp99oRUjDy~oZSBX68aDgEx6jjB2-4aeF43TljjwPxtZt4wfEQfzuKWfQEBG-6nVqa30s1bzcApihhsrqVBpJZDBg4XkFwWwTqKZ7hNTrm6OeTfu2trsNRZ3dnJKZKF8iTGCor4eYVWj3jZBp1IOQvGM7D3TUo~4eXtXmt59Dn6tv2x5NNulKqSx1NAcvvYQO8wFxekyXDwpxbnt-J~SCI~qTXaZ7rl1Y~IPBjcBdeL2BeyEJGSgCz28cdo7OZWvIlf9KYQu7azHzK79q~I~ZgeAtPH2Wr~N8ZMuIg-2n~JWGdgUiArZGQhnLznKoKOw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[Ca++]i-buffering causes a redistribution of F-actin and talin in PMNs.
PMNs were either loaded with quin2/AM (D-F) or not (A-C), then prepared for immunofluorescence as described. Samples were incubated in 5 μg/mL of a monoclonal antibody to talin, rinsed, and then stained with a TRITC-conjugated secondary antibody and FITC-conjugated phalloidin to visualize F-actin. In control cells (A-C), talin (C) is found colocalized with F-actin (B) predominately at the leading edge of motile cells. In contrast, when cells are [Ca++]i-buffered both talin (F) and F-actin (E) are found at the rear of cells as well as in the leading lamella if one exists. DIC images are shown (A, D). Bar = 10 μm.
