Fig. 8.
5 integrin colocalizes with a lipid marker of the ERC.
PMNs were incubated with Cy3-VC5 and MOPC21 for 30 minutes on ice, then labeled with C6-NBD-gal. Cells were washed well, incubated at 37°C for 30 minutes, then plated onto fibronectin-coated coverslip dishes. PMNs were incubated in the presence (D-F) or absence (A-C) of fMLP before fixation with 2% paraformaldehyde. Cy3-VC5 (B, E) is seen on the plasma membrane, as well as accumulated in a centrally located cluster inside the cells. The intracellular clusters of α5 colocalize with a compartment labeled with C6-NBD-gal (C, F) in both unstimulated (A-C) and stimulated (D-F) cells. DIC images are shown in (A, D). Bar = 10 μm.
