Elevated expression of MUC-1 mucin in tumor 2385.

Total RNA extracted from tumor 2385 and lymphoma cell lines OCI-Ly 3 and OCI-Ly 12 were hybridized with an MUC1 tandem repeat probe and subsequently with GAPDH to control for loading (A). Expression of MUC-1 mucin in K562, OCI-Ly 3, OCI-Ly 12, and tumor 2385 cells by flow cytometric analysis (B). Live cells were incubated either with normal mouse serum (gray line) or a MUC-1 mucin antibody (HMFG-2) (black line) and quantitated by FACS analysis. MUC-1 mucin staining is plotted as a histogram.