NBMPR-induced potentiation of Tomudex and LY231514 cytotoxicity in control murine myeloid progenitor cells.
Murine bone marrow cells were transduced with a control retroviral vector expressing only GFP. Transduced cells were treated in growth medium supplemented with 15% dialyzed FBS, myeloid cytokines, 1 μmol/L thymidine, and 100 μmol/L hypoxanthine. Varying concentrations of Tomudex (0 to 500 nmol/L) (A) or LY231514 (0 to 1000 nmol/L) (B) were added to culture media in the presence or absence of 1 μmol/L NBMPR. After 4 days of drug treatment, the volume equivalent 1 × 105 BM cells from control untreated cultures were washed and plated in 3 mL drug-free methylcellulose semisolid media. Myeloid progenitor colony counts were determined 7 days later by scoring colonies with more than 50 cells. Progenitor contents of the drug-treated cultures are expressed as a percentage of the colony number observed in untreated cultures.
