Fig. 6.
![Fig. 6. Functional expression of the IE transgene on B cells from B6 CD19-IE mice induces antigen-specific proliferation in I-E restricted CD4 T cells. / B220-positive splenocytes from B6 and B6 CD19-IE animals were sorted, irradiated, and used as APC for preactivated T-cell blasts from AD10 TCR transgenic mice. The coculture was performed in the presence of graded amounts of antigenic peptide (MCC88-103), and proliferation was measured by [3H] thymidine incorporation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2610/4/bloo00811006x.jpeg?Expires=1735862267&Signature=1hEmq-rMIxolBKJZU5783t113Hr-s~AZU9nuXK2PL8eXyX-l2aksYnDKbotDrQuGxb-kMaOOTwA4KAmH8SUiFQvPr3hO45auM4WXjFXCV7wPGXiLXC8Q1q7t9wLoMUbbO5sjrp72nOvi1A0eMuaODk6dNHP7T-5eDOviWSEEiYFMa9OJFff2vfrFmb50VlsB0oQfa5Uv474bmEYYCs8zrrPQ7AVK2zfshLsk0riS1dxDcMSmn6FaWCBsrMoxg-ZPkcFkc1e7Z19MC~QkizyYXzQ8OYhWwESvRDcAbxdT7qHG~pj-y~T8hidWyWATrZSbG05g7e2XWCkmDWuvcFbI~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional expression of the IE transgene on B cells from B6 CD19-IE mice induces antigen-specific proliferation in I-E restricted CD4 T cells.
B220-positive splenocytes from B6 and B6 CD19-IE animals were sorted, irradiated, and used as APC for preactivated T-cell blasts from AD10 TCR transgenic mice. The coculture was performed in the presence of graded amounts of antigenic peptide (MCC88-103), and proliferation was measured by [3H] thymidine incorporation.
