Characterization of fibrinogen species in peaks A and B. (A) Unreduced samples and (B) reduced samples. Fibrinogen (Fib I-2) represents the material added, and peak A and peak B represent the material eluted from the anion exchange column of Figure 1. Western blot analysis was performed with either polyclonal anti-αEC #9395 or monoclonal anti-α(603-610). Samples in the upper panel were electrophoresed on 4-15% SDS-PAGE gels; proteins in the lower panels were separated on homogeneous 12% SDS-PAGE gels. Positions of various hexamers (A) and individual chains (B) are indicated. dα and dαE refer to degraded α and αE, respectively. All designated α gene-derived species were recognized by 1D4, an antibody specific for an epitope in the center of the αC region (not shown).
