Fig. 3.
Representative standard curve for quantifying patient samples.
Patient IgH plasmids are diluted 10-fold in 7 increments (copy numbers ranging from 106 to 1) and subjected to real-time quantitative PCR in triplicate. Plasmid copy number (starting quantity) of standards is plotted against Ct (threshold cycle—cycle number at which fluorescence first rises above background) to create a standard curve for quantification of unknown samples.
