Fig. 1.
Fig. 1. Sorting criteria for CD34++CD38− , CD34+CD38+, and CD34+Lin− cells. / (A) Side (SSC) versus forward (FSC) scatter for ABM mononuclear cells. The rectangular region (R1) defines the gated population used for sorting. (B) Fluorescent intensities of CD34−FITC and CD38−PE for all cells within the gated region R1. R2 defines the gated population used for sorting the CD34+CD38+ cells, and R3 defines the gated population used for sorting the CD34++CD38− cells. (C) Fluorescent intensities of CD34−PE and CD2/CD7/CD19−FITC for all cells within the gated region R1. R4 defines the gated population used for sorting the CD34+Lin− cells. Dot plots represent the sorting criteria for a single ABM experiment, but similar plots were obtained for the other ABM experiments.

Sorting criteria for CD34++CD38− , CD34+CD38+, and CD34+Lin− cells.

(A) Side (SSC) versus forward (FSC) scatter for ABM mononuclear cells. The rectangular region (R1) defines the gated population used for sorting. (B) Fluorescent intensities of CD34−FITC and CD38−PE for all cells within the gated region R1. R2 defines the gated population used for sorting the CD34+CD38+ cells, and R3 defines the gated population used for sorting the CD34++CD38− cells. (C) Fluorescent intensities of CD34−PE and CD2/CD7/CD19−FITC for all cells within the gated region R1. R4 defines the gated population used for sorting the CD34+Lin− cells. Dot plots represent the sorting criteria for a single ABM experiment, but similar plots were obtained for the other ABM experiments.

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