Fig. 2.
Fig. 2. Intracellular detection of IL-16 in infiltrating cells into DTH footpads. / Infiltrating cells into DTH footpads were isolated 24 hours after the Ag challenge and stained intracellularly with PE-conjugated anti-IL-16 (14.1) or isotype-matched control mAb (G155-178). For blocking, PE-conjugated anti-IL-16 was preincubated with recombinant IL-16 and used for the staining. Similar results were obtained in 3 independent experiments.

Intracellular detection of IL-16 in infiltrating cells into DTH footpads.

Infiltrating cells into DTH footpads were isolated 24 hours after the Ag challenge and stained intracellularly with PE-conjugated anti-IL-16 (14.1) or isotype-matched control mAb (G155-178). For blocking, PE-conjugated anti-IL-16 was preincubated with recombinant IL-16 and used for the staining. Similar results were obtained in 3 independent experiments.

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