Kinetic analysis on the IL-16 production in DTH footpads.

Sensitized mice were challenged with mBSA into footpads, and the footpad swelling was measured at a given time (A). Then, swollen footpads were cut off, minced in cold PBS, and briefly incubated on ice. Resultant supernatant was analyzed for IL-16 by ELISA (B). Similar results were obtained in 2 independent experiments.