Production of bioactive IL-16 in DTH footpads.

Sensitized mice were challenged with mBSA into footpads, and swollen footpads were cut off 24 hours after the Ag challenge, minced in cold PBS, and briefly incubated on ice. Resultant supernatant was then analyzed for IL-16 chemoattractant activity in the presence and absence of neutralizing mAb against IL-16 (14.1) using a migration assay. The inclusion of an isotype-matched control mAb (MOPS-173) did not affect the migration assay (data not shown). Data are shown as mean ± SD of 4 mice. *indicates P < .001 compared with PBS-challenged footpads of sensitized mice and footpads of nontreated mice. **indicates P < .001 compared with the absence of anti-IL-16. Similar results were obtained in 3 independent experiments.