![Fig. 7. Inhibition of cellular infiltration into DTH footpads by anti-IL-16 treatment. / Sensitized mice were treated with neutralizing mAb against IL-16 (14.1, B) or an isotype-matched control mAb (S-S.1, A) and challenged with mBSA into footpads. Soft tissue samples from each footpad were collected 24 hours after the Ag challenge, and the tissue section was immunohistochemically analyzed using anti-CD4 (GK1.5, A and B). The section was also stained with anti-CD8 and antimacrophage mAbs (53.6.7 and F4/80, respectively). No staining was observed when isotype-matched control mAbs (R35-95 and R35-38) were used (data not shown). Positively stained cells were counted in 10 randomly selected fields (each [100 μm]2) (C). Data are shown as mean ± SD of 5 anti-IL-16–treated mice and 8 control mAb-treated mice. *indicates P < .01 compared with control mAb. Similar results were obtained in 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/9/10.1182_blood.v95.9.2869.009k18_2869_2874/5/bloo00918007ay.jpeg?Expires=1735851095&Signature=YEQSA7CYiyhn6AxF8XzDxRUBf6-LC4T2VnKABreOtJSyu-K0bHfIxXpBALt~dnXpps5754FRdHHPwUNiRpVE5bLrt4QDSZPMP2ryD6cJowOpcrS9GuL~4Yf3Eg4WlXt5Vd0CgJL~xUHjopcpt2PoClXHJBUx9l1DRZ0dznmWoiXliwmfzyHqwP3e3AbxyDHkW5cA-gVeqx-gGqA4R5~Jxcs~Vyp9kPj0aMGwsd86bxjkvWJlJ7qBoIcViBYBW0xJkstrwf2WQUS8bN26Ehfx8CLptOmC9GbI0pd4vAwJA7bOO3vfnsv3Wn7qZ8e1uSaieYA4VTibVKkVr3Fa12pGnA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of cellular infiltration into DTH footpads by anti-IL-16 treatment.
Sensitized mice were treated with neutralizing mAb against IL-16 (14.1, B) or an isotype-matched control mAb (S-S.1, A) and challenged with mBSA into footpads. Soft tissue samples from each footpad were collected 24 hours after the Ag challenge, and the tissue section was immunohistochemically analyzed using anti-CD4 (GK1.5, A and B). The section was also stained with anti-CD8 and antimacrophage mAbs (53.6.7 and F4/80, respectively). No staining was observed when isotype-matched control mAbs (R35-95 and R35-38) were used (data not shown). Positively stained cells were counted in 10 randomly selected fields (each [100 μm]2) (C). Data are shown as mean ± SD of 5 anti-IL-16–treated mice and 8 control mAb-treated mice. *indicates P < .01 compared with control mAb. Similar results were obtained in 2 independent experiments.
