Decreased production of MIP-1 in DTH footpads by anti-IL-16 treatment.

Sensitized mice were challenged with mBSA into footpads, and swollen footpads were cut off, minced in cold PBS, and briefly incubated on ice. Resultant supernatant was then analyzed for MIP-1α by ELISA (A). Sensitized mice were treated with neutralizing mAb against IL-16 (14.1) or an isotype-matched control mAb (S-S.1) and challenged with mBSA into footpads. Swollen footpads were then cut off 12 hours after the Ag challenge, minced in cold PBS, and briefly incubated on ice, and resultant supernatant was analyzed for MIP-1α by ELISA (B).