Fig. 1.
Combined DIC and fluorescence image (A), DIC image (B), and fluorescence images (A′, B′) of living neutrophils.
Cells were labeled at 4°C with anti-CD43 mAbs (A, A′) or anti-CD11b mAbs (B, B′) and TRITC-conjugated F(ab′)2 fragments of secondary antibodies. They were analyzed at 37°C as described in “Materials and methods”. (A, A′) Polarized, locomoting neutrophils with CD43 caps located at the uropods. (B, B′) Round, stationary neutrophils with uniformly patched CD11b. Scale bar = 10 μm. (Panel A, the top left corner cell is round, nonpolarized, and nonmotile.)
