CD43 distribution in neutrophils developing locomotory activity on fibronectin.

(A) Neutrophils were allowed to settle on fibronectin for 5 minutes at 37°C and were stimulated by fMLP for 30, 60, and 120 seconds. Cells were then fixed and labeled for CD43. (B) After 5 minutes of locomotion on fibronectin, at 37°C, cells were fixed and double labeled for CD43 extracellular domain (Alexa 488) and for CD43 intracellular domain (rhodamine). Scale bars = 10 μm.