Time series of neutrophils developing or losing polarity and locomotor activity on a fibronectin-coated surface.

Cells were labeled for CD43 (with Fab fragments of primary and secondary antibodies) at 4°C, then allowed to settle on the fibronectin-coated surface for 5 minutes at 25°C. fMLP (10 nM) was applied and fluorescence images were acquired every 10 seconds. (A) Images have been selected at 60-second intervals. The lines in the last panel represent the tracks of the cell centroids during the 320 seconds of stimulation. (B) The first image represents the uropod of a neutrophil after 5 minutes of locomotion. The cells were then washed in a medium without fMLP. In B, images have been selected at the indicated times (0, 60, 140, and 190 seconds). Scale bar = 10 μm.