Fig. 2.
Effects of endogenous NGF neutralization on the basal growth of HHV-8+ and HHV-8− B-lymphoma cell lines.
The figure depicts untreated cells (•), cells treated with 30 μg/mL anti-NGF Ab at 0 hour (○), and cells treated with 30 μg/mL anti-NGF Ab at 0 hour and 48 hours (▵). Cells were seeded in duplicate at (A, C) 2 × 105/mL or (B) 5 × 105/mL at 0 hour. The number of viable cells in each culture was determined daily using the trypan blue dye exclusion method. The values refer to the mean of each duplicate. In all cases, the SD was less than 10% of the mean. Similar results were obtained in 3 separate experiments.