Effects of endogenous NGF neutralization by means of anti-NGF antibodies or NGF antisense oligonucleotides on3H-thymidine incorporation by BCBL-1 cells.
(A) Cells were treated with 30 μg/mL anti-NGF Ab at 0 hour (□) and with 30 μg/mL anti-NGF Ab at 0 and 48 hours (▪). (B) Cells were treated with 2 μmol/L (▵) or 10 μmol/L (▴) NGF antisense oligonucleotides and with 10 μmol/L (○) control random-sequence oligonucleotides. Cells were seeded in quadruplicate at 1 × 105 cells/mL in 96-well plates.3H-thymidine (0.037 MBq/well) was added in the final 12 hours of incubation for each time point. Results were calculated as the mean 3H-thymidine incorporation of quadruplicate cultures and expressed as the percentage of untreated controls. Similar results were obtained in 3 separate experiments.
