Confocal fluorescence micrographs of BCBL-1 cells cultured in the absence or presence of anti-NGF antibodies.

Untreated cells were observed at (A) 24 hours, (C) 48 hours, and (E) 72 hours. Cells were treated with 30 μg/mL anti-NGF Ab at 0 hour and observed at (B) 24 hours, (D) 48 hours, and (F) 72 hours. (G) Cells were treated with 30 μg/mL anti-NGF Ab at 0 and 48 hours and observed at 72 hours. To observe nuclei, cells were stained with propidium iodide. Apoptosis was evaluated using morphological parameters such as chromatin condensation and nuclear fragmentation (seen in dense black). (Original magnification ×40.)