Fig. 5.
Effects of TPA plus hr-NGF or anti-NGF antibodies on BCBL-1.
(A) Cell growth curves and (B) the percentage of apoptotic cells is depicted. Cells were seeded in duplicate at 2 × 105/mL. The figure depicts untreated cells (•), cells treated with 20 ng/mL TPA at 0 hour (▴), cells treated with TPA plus 5 ng/mL hr-NGF at 0 hour (▵), and cells treated with TPA plus 30 μg/mL anti-NGF Ab at 0 and 24 hours (○). The number of viable cells in each culture was determined daily using the trypan blue dye exclusion method. The values refer to the mean of each duplicate. In all cases, the SD was less than 10% of the mean. Similar results were obtained in 3 separate experiments.
